COMPOSITION AND METHODS FOR RT-PCR COMPRISING AN ANIONIC POLYMER

• Main Authors: FANG, NAN, MISSEL, ANDREAS
• Format: Patent
• Language: English; French
• Published: 21.02.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention is in the fields of molecular biology. The present invention is directed to novel compositions, methods and kits useful for the generation of nucleic acids from an RNA template and further nucleic acid replication. Specifically, the invention is directed to the generation and amplification of nucleic acids by reverse transcriptase-polymerase chain reaction. La présente invention appartient au domaine de la biologie moléculaire. La présente invention concerne des nouvelles compositions, de nouveaux procédés et de nouvelles trousses utiles pour la génération d'acides nucléiques à partir d'une matrice d'ARN ainsi qu'une réplication de l'acide nucléique. De façon spécifique, l'invention concerne la génération et l'amplification d'acides nucléiques par une réaction en chaîne de la polymérase après transcription inverse.