METHODS FOR DEVELOPING ANTIGEN-SPECIFIC ANTIBODY-PRODUCING CELL LINES AND MONOCLONAL ANTIBODIES

Disclosed are methods for producing class-switched, affinity-matured antibodies which include enriching an immunized cell population tor GL7-positive cells and activating the enriched cells. The methods may be used to improve the efficiency of obtaining immortalized antigen-specific plasma cells or...

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Bibliographic Details
Main Authors KRAVITZ, RACHEL, SCHRAM, BRIAN
Format Patent
LanguageEnglish
French
Published 27.12.2012
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CARE OF BIRDS, FISHES, INSECTS
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW BREEDS OF ANIMALS
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
SPIRITS
TRAPPING
VINEGAR
WINE
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Summary:Disclosed are methods for producing class-switched, affinity-matured antibodies which include enriching an immunized cell population tor GL7-positive cells and activating the enriched cells. The methods may be used to improve the efficiency of obtaining immortalized antigen-specific plasma cells or to improve the quality of molecularly cloned Ig heavy and light chains. La présente invention concerne des procédés de production d'anticorps à commutation isotypique, et à maturation d'affinité comprenant l'enrichissement d'une population de cellules immunisées en cellules GL7 positives et l'activation des cellules enrichies. Les procédés peuvent être utilisés pour améliorer l'efficacité d'obtention de plasmocytes immortalisés spécifiques à un antigène ou pour améliorer la qualité de chaînes lourdes et légères d'Ig clonées de manière moléculaire.
Bibliography:Application Number: WO2012US43962