PROBE AND PRIMER SEQUENCES FOR DETECTION OF HEPATITIS B VIRUS BY REAL-TIME PCR, PCR REACTION MIXTURE AND KITS THEREOF

• Main Authors: SUBBARAO, PILLARISETTI, VENKATA, MANOJ, MULAKKAPURATH, NARAYANAN, NAIR, BHASKARAN, CHANDRASEKHAR, KUDVA, AVINASH, KUNDADKA, MANJULA, JAGANNATH
• Format: Patent
• Language: English; French
• Published: 07.09.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The present disclosure relates to method of determining presence and quantification of Hepatitis B virus (HBV) nucleic acids in biological samples. The present disclosure also discloses probe and primer sequences set forth as SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3 for the detection of Hepatitis B Virus. Further, the disclosure provides for PCR reaction mixture and kit for said detection and optional quantification. La présente invention concerne un procédé de détermination de la présence et de la quantification d'acides nucléiques du virus de l'hépatite B (HBV) dans des échantillons biologiques). L'invention décrit également des séquences de sondes et d'amorces représentées par SEQ ID No.1, SEQ ID No.2 et SEQ ID No.3 pour la détection du virus de l'hépatite B. En outre, l'invention concerne un mélange pour une réaction de PCR et un kit pour ladite détection et la quantification éventuelle.