STIMULI-RESPONSIVE POLYMER DIAGNOSTIC ASSAY COMPRISING MAGNETIC NANOPARTICLES AND CAPTURE CONJUGATES

• Main Authors: NEHILLA, BARRETT, J, STAYTON, PATRICK, S, LAI, JRIUAN, SRINIVASAN, SELVI
• Format: Patent
• Language: English; French
• Published: 18.05.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NANOTECHNOLOGY; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SEPARATION; SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION; TECHNICAL SUBJECTS COVERED BY FORMER USPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; TESTING; TRANSPORTING; VINEGAR; WINE

The present disclosure utilizes the aggregation of stimuli-responsive polymers to isolate a diagnostic target (e.g., an antigen) from a solution using magnetophoresis. Isolating the diagnostic target provides a route to identify the presence of the diagnostic target in the solution. For example, utilized is a two-component assay (eg sandwich assay) comprising a magnetic nanoparticle functionalized with a stimuli-responsive polymer, and a capture molecule attached to a second stimuli-responsive polymer. La présente invention fait appel à l'agrégation de polymères réagissant aux stimuli pour isoler une cible de diagnostic (par ex. un antigène) d'une solution en utilisant la magnétophorèse. L'isolation de la cible de diagnostic ouvre la voie à l'identification de la présence de la cible de diagnostic dans la solution. L'invention utilise par exemple une technique à deux composants (par ex. une technique en sandwich) comportant une nanoparticule magnétique fonctionnalisée au moyen d'un polymère réagissant aux stimuli et une molécule de capture liée à un deuxième polymère réagissant aux stimuli.