IDENTIFICATION OF CENTROMERE SEQUENCES USING CENTROMERE-ASSOCIATED PROTEINS AND USES THEREOF

• Main Authors: ZIELER, HELGE, PREUSS, DAPHNE, COPENHAVER, GREGORY, P
• Format: Patent
• Language: English; French
• Published: 28.06.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention is directed to methods of centromere discovery using centromere- associated proteins in a variety of experimental formats. The methods of the invention can be used on any organism, and include using Call,Cbfl, Cbf3, Cbf5, CenH3 (Cenp-A), Cenp-B, Cenp-C, Cenp-D, Cenp-E, Cenp-F, Cenp-G, Cenp-H, Cenp-I, Cenp-K, Cenp-L, Cenp-M, Cenp-N, Cenp-O, Cenp-P, Cenp-Q, Cenp-R, Cenp-S, Cenp-T, Cenp-U, Cenp-V, Cenp-W, Chdl, Chpl, cohesin, condensin, Dnmt3b, Fact, Gcn5p, H2A.Z, Haspin, Hjurp, HP1, Hst4, Imal, Incep, Ino80, Kms2, Knl-2, Mif2, Mis6, Np95, Pich, Sadl, Scm3, Shugoshin, Sim3, Skpl, Sororin, Survivin, Tas3, ZW10, and homologs thereof to identify centromere sequences. The invention is also directed to artificial chromosomes comprising centromeres made according to the methods of the invention, as well as to cells comprising such artificial chromosomes. La présente invention concerne des procédés de découverte de centromère utilisant des protéines associées aux centromères dans différents formats expérimentaux. Les procédés de l'invention peuvent être utilisés sur un organisme quelconque, et comprennent l'utilisation de Call, Cbfl, Cbf3, Cbf5, CenH3 (Cenp-A), Cenp-B, Cenp-C, Cenp-D, Cenp-E, Cenp-F, Cenp-G, Cenp-H, Cenp-I, Cenp-K, Cenp-L, Cenp-M, Cenp-N, Cenp-O, Cenp-P, Cenp-Q, Cenp-R, Cenp-S, Cenp-T, Cenp-U, Cenp-V, Cenp-W, Chdl, Chpl, cohésine, condensine, Dnmt3b, Fact, Gcn5p, H2A.Z, haspine, Hjurp, HP1, Hst4, Imal, Incep, Ino80, Kms2, Knl-2, Mif2, Mis6, Np95, Pich, Sadl, Scm3, shugoshine, Sim3, Skpl, sororine, survivine, Tas3, ZW10, et des homologues de ceux-ci pour identifier des séquences de centromère. L'invention concerne en outre des chromosomes artificiels comprenant des centromères produits selon les procédés de l'invention, ainsi que des cellules comprenant de tels chromosomes artificiels.