SCAFFOLD-BASED POLYMERASE ENZYME SUBSTRATES

• Main Authors: SHEN, GENE, SEBO, LUBOMIR, YUE, STEPHEN, FEDEROV, ANDREI, SUN, DAVID, BROGLEY, LOUIS
• Format: Patent
• Language: English; French
• Published: 02.08.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HETEROCYCLIC COMPOUNDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates. L'invention concerne une nouvelle classe de substrats de type enzyme polymérase marquée à base d'un échafaudage. Les substrats de type enzyme polymérase présentent un noyau ou un échafaudage multivalent auquel sont attachés des fragments de colorant fluorescent et des fragments de nucléoside-phosphate. Les substrats de type enzyme polymérase présentent de multiples fragments de colorant fluorescent et/ou de multiples fragments de nucléoside-phosphate. Des noyaux multivalents préférés comprennent des fragments aromatiques à six chaînons trifonctionnels. L'invention concerne également des procédés et des kits de séquençage comprenant des substrats de type enzyme polymérase marquée à base d'un échafaudage.