ARTIFICIAL CELLULOSOME AND THE USE OF THE SAME FOR ENZYMATIC BREAKDOWN OF RESILIENT SUBSTRATES

• Main Authors: HORNBURG, DANIEL, KRAUSS, JAN, KOECK, DANIELA, SCHWARZ, WOLFGANG H, SCHULTE, LOUIS-PHILIPP, ZVERLOV, VLADIMIR V
• Format: Patent
• Language: English; French
• Published: 26.01.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGYGENERATION, TRANSMISSION OR DISTRIBUTION; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

The present invention relates to an in vitro produced, artificial cellulosome for enzymatic breakdown of resilient substrates. In particular, the present invention provides a complex having an increased activity on resilient substrates, such as crystalline cellulose. The in vitro formed complex comprises a backbone scaffold having at least four binding sites capable of binding the enzyme components, whereby at least two of the binding sites have essentially the same binding specificity; and at least three different enzyme components being randomly bound to the at least four binding sites. Method for preparing the complex and uses of the same for enzymatic breakdown of resilient substrates are also provided. La présente invention concerne un cellulosome artificiel produit in vitro, un cellulosome artificiel pour la décomposition enzymatique de substrats résilients. En particulier, la présente invention concerne un complexe ayant une activité augmentée sur des substrats résilients, tels que la cellulose cristalline. Le complexe formé in vitro comprend un échafaudage de squelette ayant au moins quatre sites de liaison capables de se lier aux composants d'enzyme, de telle manière qu'au moins deux des sites de liaison aient essentiellement la même spécificité de liaison ; et au moins trois composants d'enzyme différents étant liés de façon aléatoire aux au moins quatre sites de liaison. La présente invention concerne en outre un procédé pour préparer le complexe et des utilisations de celui-ci pour la décomposition enzymatique de substrats résilients.