FILLER FOR AFFINITY CHROMATOGRAPHY

• Main Authors: SHIOTANI, TOMONORI, IKKYU, KAZUHIRO, ABE, TAKAYOSHI, OKANO, YUSUKE, KAWAI, HIROSHI, TAMORI, KOUJI, HYUGAJI, SATOSHI, WANG, YONG, MOMIYAMA, MASAKI
• Format: Patent
• Language: English; French; Japanese
• Published: 13.10.2011
• Subjects: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; MEASURING; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; SEPARATION; TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION; TECHNICAL SUBJECTS COVERED BY FORMER USPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TESTING; THEIR PREPARATION OR CHEMICAL WORKING-UP; THEIR RELEVANT APPARATUS; TRANSPORTING

The disclosed filler for affinity chromatography has a high dynamic binding capacity for proteins, excellent alkali resistance, and excellent storage stability. Said filler contains porous particles comprising a copolymer of: between 40 and 99.5 mass parts of a methacryloyl-group-containing vinyl monomer (M-1) that contains a hydroxide group but no epoxy groups; between 0.5 and 30 mass parts of an epoxy-group-containing vinyl monomer (M-2); between 0 and 59.5 mass parts of a methacryloyl-group-containing vinyl monomer (M-3) other than (M-1) and (M-2); and between 0 and 25 mass parts of a vinyl monomer (M-4) other than (M-1), (M-2), and (M-3). The amounts of (M-1), (M-2), (M-3), and (M-4) sum to 100 mass parts. The disclosed filler also contains: ring-opened epoxy groups obtained by ring-opening epoxy groups in the aforementioned copolymer; and ligands bound to the porous particles. L'invention divulgue une charge pour la chromatographie d'affinité qui a une capacité de liaison dynamique élevée pour les protéines, une excellente résistance aux alcalins et une excellente stabilité au stockage. Ladite charge contient des particules poreuses comprenant un copolymère de : entre 40 et 99,5 parties en masse d'un monomère de vinyle contenant un groupement méthacryloyle (M-1) qui contient un groupement hydroxyde mais pas de groupements époxy ; entre 0,5 et 30 parties en masse d'un monomère de vinyle contenant un groupement époxy (M-2) ; entre 0 et 59,5 parties en masse d'un monomère de vinyle contenant un groupement méthacryloyle (M-3) autre que (M-1) et (M-2) ; et entre 0 et 25 parties en masse d'un monomère de vinyle (M-4) autre que (M-1), (M-2) et (M-3). Les quantités de (M-1), (M-2), (M-3) et (M-4) totalisent 100 parties en masse. La charge divulguée contient aussi : des groupements époxy à cycle ouvert obtenus par ouverture de cycle des groupements époxy dans le copolymère ci-avant mentionné ; et des ligands liés aux particules poreuses.