METHODS AND COMPOSITIONS FOR AMPLIFYING TARGET SEQUENCES FROM NUCLEIC ACID SAMPLES

The present invention concerns in general PCR reaction mixtures comprising a mixture of hot- start primers and non-hot-start primers for a given target sequence, including multiplex PCR reaction mixtures; methods utilizing same for detection of one or more target polynucleotide sequences; and kits c...

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Bibliographic Details
Main Authors TZUBERY, TZVI, GASSEL, ARYEH, ARIELI, BOAZ, GASSEL, RAPHAEL, GLASNER, SHIRA, TAL, MAOZ, SALOMON, TAL
Format Patent
LanguageEnglish
French
Published 21.04.2011
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:The present invention concerns in general PCR reaction mixtures comprising a mixture of hot- start primers and non-hot-start primers for a given target sequence, including multiplex PCR reaction mixtures; methods utilizing same for detection of one or more target polynucleotide sequences; and kits comprising same. La présente invention concerne de manière générale des mélanges réactionnels de PCR qui comprennent un mélange d'amorces à démarrage à chaud et d'amorces non à démarrage à chaud pour une séquence cible donnée, y compris des mélanges réactionnels de PCR multiplex ; des procédés les utilisant pour la détection d'une ou de plusieurs séquences polynucléotidiques cibles ; et des kits les comprenant.
Bibliography:Application Number: WO2010IB54613