RNA SYNTHESIS - PHOSPHORAMIDITES FOR SYNTHETIC RNA IN THE REVERSE DIRECTION, AND APPLICATION IN CONVENIENT INTRODUCTION OF LIGANDS, CHROMOPHORES AND MODIFICATIONS OF SYNTHETIC RNA AT THE 3' - END

The present invention relates to novel phosphoramidites, A-n-bz, C-n-bz, C-n-ac, G-n-ac and U are produced with an HPLC purity of greater than 98% and 31P NMR purity greater than 99%. A novel process of reverse 5'? 3' directed synthesis of RNA oligomers has been developed and disclosed. Us...

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Bibliographic Details
Main Authors SRIVASTAVA, NAVEEN, P, PANDEY, DIVYA, BAJPAI, SATYA, P, SRIVASTAVA, SURESH, C
Format Patent
LanguageEnglish
French
Published 02.09.2010
Subjects
CHEMISTRY
DERIVATIVES THEREOF
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
SUGARS
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Summary:The present invention relates to novel phosphoramidites, A-n-bz, C-n-bz, C-n-ac, G-n-ac and U are produced with an HPLC purity of greater than 98% and 31P NMR purity greater than 99%. A novel process of reverse 5'? 3' directed synthesis of RNA oligomers has been developed and disclosed. Using that method demonstrated high quality RNA synthesis with coupling efficiency approaching 99%. La présente invention concerne de nouveaux phosphoramidites, A-n-bz, C-n-bz, C-n-ac, G-n-ac et U qui sont produits avec une pureté par CLHP supérieure à 98 % et une pureté par RMN au 31P supérieure à 99 %. Un nouveau procédé de synthèse antisens dirigée de 5' ? 3' d'oligomères d'ARN a été développé et présenté. L'utilisation de ce procédé a montré une qualité de synthèse d'ARN élevée avec une efficacité de couplage approchant les 99 %.
Bibliography:Application Number: WO2009US05063