DEPROTECTION OF SYNTHETIC OLIGONUCLEOTIDES USING AQUEOUS ETHANOLAMINE

• Main Authors: GOSSELIN, FRANCIS, GOWANI, AMRAN
• Format: Patent
• Language: English; French
• Published: 19.11.2009
• Subjects: CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; DERIVATIVES THEREOF; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; SUGARS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE

This invention concerns an efficient and safer (easy to handle and environmentally less hazardous) protocol for cleavage and deprotection of synthetic oligonucleotides utilizing ethanolamine. Specifically, the present invention features a method for 1) the removal of amide protecting groups from nucleobases and 2) cleavage of the synthetic oligonucleotides from their solid support allowing the isolation and subsequent purification of said oligonucleotides. Cette invention concerne un protocole efficace et plus sûr (facile à gérer et moins nocif pour l'environnement) utilisant de l'éthanolamine pour le clivage et la déprotection d'oligonucléotides de synthèse. Plus particulièrement, l'invention concerne une méthode permettant: 1) l'élimination de groupes de protection amide de nucléobases; et 2) le clivage d'oligonucléotides de synthèse de leur support solide assurant ainsi l'isolement et la purification subséquente des oligonucléotides.