COMPOSITIONS AND METHODS OF USING RNA INTERFERENCE FOR CONTROL OF NEMATODES

• Main Authors: MC CAIG, BONNIE, MC MILLAN, JOHN, PEIFENG, REN, MOTYKA, SHAWN, LAWRENCE WINFIELD, TALTON
• Format: Patent
• Language: English; French
• Published: 05.11.2009
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; TRAPPING; VINEGAR; WINE

The present invention provides double stranded RNA compositions and transgenic plants capable of inhibiting expression of essential genes in parasitic nematodes, and methods associated therewith. Specifically, the invention relates to the use of RNA interference to inhibit expression of a target essential nematode gene, which is a nematode innexin -like, pas-1, tcp-1, snurportin-1 like, pol delta S, prs-4, rtp-1 or rpn-5 gene, and relates to the generation of plants that have increased resistance to parasitic nematodes. La présente invention porte sur des compositions d'ARN double brin et sur des plantes transgéniques capables d'inhiber l'expression de gènes essentiels dans des nématodes parasitaires, et sur des procédés associés à celles-ci. De façon spécifique, l'invention porte sur l'utilisation d'une interférence d'ARN pour inhiber l'expression d'un gène de nématode essentiel cible, qui est un gène de type innexine, pas-1, tcp-1, snurportine-1, pol delta S, prs-4, rtp-1 ou rpn-5 de nématode et porte sur la génération de plantes qui ont une résistance accrue envers les nématodes parasitaires.