DEPROTECTION OF OLIGONUCLEOTIDES THAT CONTAIN ONE OR MORE RIBONUCLEOTIDES

This invention concerns an efficient, friendly and safer (easy to handle and environmentally less hazardous) protocol for deprotection of silyl protecting groups during the synthesis of oligonucleotides containing one or more ribonucleotides. This protocol can be applied to a pure RNA molecule or a...

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Bibliographic Details
Main Authors CVETOVICH, RAYMOND, ZEWGE, DANIEL, SIDLER, DANIEL, R
Format Patent
LanguageEnglish
French
Published 08.04.2010
Subjects
CHEMISTRY
CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS
DERIVATIVES THEREOF
GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC
GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
SUGARS
TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE
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Summary:This invention concerns an efficient, friendly and safer (easy to handle and environmentally less hazardous) protocol for deprotection of silyl protecting groups during the synthesis of oligonucleotides containing one or more ribonucleotides. This protocol can be applied to a pure RNA molecule or a molecule that has combinations of RNA and DNA, composed of pure or mixtures of modified, unmodified and abasic oligonucleotides. Specifically, the present invention features a method for the removal of protecting groups from 2' -hydroxyl (2' -OH) allowing the subsequent purification and isolation of oligonucleotides comprising of one or more ribonucleotides. La présente invention concerne un protocole efficace, convivial et plus sûr (facile à manipuler et moins dangereux pour l'environnement) pour la déprotection de groupements protecteurs silylés pendant la synthèse d'oligonucléotides contenant un ou plusieurs ribonucléotides. Ce protocole peut être appliqué à une molécule d'ARN pur ou à une molécule possédant des associations d'ARN et ADN, constituée d'oligonucléotides purs ou de mélanges d'oligonucléotides modifiés, non modifiés et abasiques. Spécifiquement, la présente invention concerne un procédé pour l'élimination des groupements protégeant le groupe 2-hydroxyle (2-OH), permettant la purification et l'isolement ultérieurs des oligonucléotides comprenant un ou plusieurs ribonucléotides.
Bibliography:Application Number: WO2009US35240