MODULATION OF ENDOGENOUS AMPK LEVELS FOR THE TREATMENT OF OBESITY

• Main Authors: SASMAL, PRADIP KUMAR, HASHAM, SUMERA NIKHAT, POTLURI, VIJAY KUMAR, CHAKRABARTI, RANJAN, DAS, SAIBAL KUMAR, IQBAL, JAVED, MISRA, PARIMAL, MADHURAREKHA, CHINNABOINA
• Format: Patent
• Language: English; French
• Published: 30.12.2009
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

A method for identifying an AMPK activator is provided. The method includes providing a sample of cells that include AMPK and perilipin, providing a sample of an AMPK activator-candidate, contacting the cell sample and the activator-candidate, and measuring a quantitative indicator of the AMPK activation within the cell sample after the contacting step. L'invention porte sur un procédé pour identifier un activateur de AMPK. Le procédé comprend les opérations consistant à se procurer un échantillon de cellules qui comprennent AMPK et de la périlipine, se procurer un échantillon d'un candidat activateur de AMPK, mettre en contact l'échantillon de cellules et le candidat activateur, et mesurer un indicateur quantitatif de l'activation d'AMPK à l'intérieur de l'échantillon de cellule après l'étape de mise en contact.