HOST CELLS AND METHODS FOR PRODUCING 3-METHYL-2-BUTEN-1-OL, 3-METHYL-3-BUTEN-1-OL, AND 3-METHYL-BUTAN-1-OL

• Main Authors: CHOU, HOWARD H, KEASLING, JAY D
• Format: Patent
• Language: English; French
• Published: 08.01.2009
• Subjects: ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE ORUNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FIRELIGHTERS; FUELS; FUELS NOT OTHERWISE PROVIDED FOR; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; LIQUEFIED PETROLEUM GAS; LUBRICANTS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NATURAL GAS; PEAT; PETROLEUM, GAS OR COKE INDUSTRIES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGYGENERATION, TRANSMISSION OR DISTRIBUTION; SPIRITS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BYSUBCLASSES C10G, C10K; TECHNICAL GASES CONTAINING CARBON MONOXIDE; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

The invention provides for a method for producing a 5-carbon alcohol in a genetically modified host cell. In one embodiment, the method comprises culturing a genetically modified host cell which expresses a first enzyme capable of catalyzing the dephosphorylation of an isopentenyl disphosphate (EPP) or dimethylallyl diphosphate (DMAPP), such as a Bacillus subtilis phosphatase (YhfR), under a suitable condition so that 5-carbon alcohol is 3-methyl-2-buten-1-ol and/or 3-methyl-3-buten-1-ol is produced. Optionally, the host cell may further comprise a second enzyme capable of reducing a 3-methyl-2-buten-1-ol to 3-methyl-butan-1-ol, such as a reductase. La présente invention porte sur un procédé de fabrication d'un alcool à 5-carbones dans une cellule hôte modifiée génétiquement. Dans un mode de réalisation, le procédé comprend la culture d'une cellule hôte modifiée génétiquement qui exprime une première enzyme capable de catalyser le déphosphorylation d'un isopentényl diphosphate (EPP) ou d'un diméthylallyl diphosphate (DMAPP), tel qu'une Bacillus subtilis phosphatase (YhfR), dans une condition appropriée pour qu'un alcool à 5-carbones qui est le 3-méthyl-2-butén-1-ol et/ou le 3-méthyl-3-butén-1-ol soit produit. Facultativement, la cellule hôte peut en outre comprendre une seconde enzyme capable de réduire un 3-méthyl-2-butén-1-ol en 3-méthyl-butan-1-ol, telle qu'une réductase.