NOVEL HIGHLY ACTIVE MODIFIED TYPE S-HYDROXYNITRILE LYASE

• Main Authors: SEMBA, HISASHI, ICHIGE, EITA
• Format: Patent
• Language: English; French; Japanese
• Published: 10.04.2008
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a novel highly active modified type S-hydroxynitrile lyase (SHNL). More particularly, the invention relates to the novel highly active modified type SHNL which can be obtained by substituting the amino acids at specific sites (at the 14th, 44th, 66th, 94th, 103rd, 118th, 122nd, 125th, 127th, 129th, 147th, 148th, 152nd, 212th and 216th positions) or inserting an amino acid at a specific site (between the 128th and 129th residues) in the amino acid sequence (SEQ ID NO: 2) of a naturally occurring type SHNL. L'invention concerne une S-hydroxynitrile lyase (SHNL) modifiée et hautement active inédite. L'invention concerne, plus particulièrement, la SHNL modifiée et hautement active inédite, qui peut être obtenue par substitution des acides aminés présents sur des sites bien précis (en 14ème, 44ème, 66ème, 94ème, 103ème, 118ème, 122ème, 125ème, 127ème, 129ème, 147ème, 148ème, 152ème, 212ème et 216ème position) ou par insertion d'un acide aminé sur un site précis (entre le 128ème et le 129ème résidu) dans la séquence d'acides aminés (SEQ ID NO : 2) d'une SHNL naturelle.