POLYSACCHARIDE TRANSFERASE

• Main Authors: HRMOVA, MARIA, FINCHER, GEOFFREY, BRUCE
• Format: Patent
• Language: English; French
• Published: 31.01.2008
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; POLYSACCHARIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

The present invention is predicated on the discovery that a polysaccharide transferase purified from barley seedlings can catalyze the formation of covalent bonds between different polysaccharides, including between xyloglucans and cellulose, and between xyloglucans and (1,3;1,4) -ß-D-glucans. The present invention thus provides, among other things, an isolated or substantially purified polysaccharide transferase, wherein said polysaccharide transferase is capable of catalyzing the formation of a covalent bond between a donor polysaccharide and an acceptor polysaccharide; or a functionally active fragment or variant of said polysaccharide transferase. La présente invention se base sur la découverte selon laquelle une polysaccharide transférase purifiée à partir de semences d'orge peut catalyser la formation de liaisons covalentes entre différents polysaccharides, y compris entre des xyloglucanes et la cellulose, et entre des xyloglucanes et des (1,3;1,4)-ß-D-glucanes. La présente invention propose ainsi, entre autres, une polysaccharide transférase isolée ou substantiellement purifiée, ladite polysaccharide transférase étant capable de catalyser la formation d'une liaison covalente entre un polysaccharide donneur et un polysaccharide accepteur; ou un fragment fonctionnellement actif ou une variante de ladite polysaccharide transférase.