METHODS OF DETECTING ROOT KNOT NEMATODES

The present invention relates to a method method of testing a sample for the presence of a Meloidogyne nematode, said method comprising the step of analyzing the nucleic acid in said sample for the presence of an rRNA gene of a Meloidogyne nematode, or the transcription product thereof, or a fragmen...

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Main Authors KARSSEN, GERRIT, HEKMAN, HENRI, LANDEWEERT, RENSKE, VEENHUIZEN, PETRUS, THEODORUS, MARIA, BAKKER, JAAP, HELDER, JOHANNES, HOLTERMAN, MARTIJN, HERMANUS, MARIA, VAN DEN ELSEN, SVEN, JOHANNES, JOSEPHUS
Format Patent
LanguageEnglish
French
Published 24.04.2008
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to a method method of testing a sample for the presence of a Meloidogyne nematode, said method comprising the step of analyzing the nucleic acid in said sample for the presence of an rRNA gene of a Meloidogyne nematode, or the transcription product thereof, or a fragment thereof, comprising at least one single nucleotide polymorphism (SNP) and/or at least one oligonucleotide polymorphism (OP) that is essentially unique on phylum level to a specific group of Meloidogyne nematode species of which said Meloidogyne nematode is a member, wherein said step of analyzing said nucleic acid involves a nucleic acid hybridization assay, and wherein said group comprises (a) the species M. chitwoodi, M. fallax </i L'invention porte sur une méthode de vérification dans un échantillon de la présence d'un nématode Méloïdogyne consistant à analyser l'acide nucléique de l'échantillon pour y rechercher la présence d'un gène d'ARNr de nématode Méloïdogyne ou son produit de transcription, ou l'un de ses fragments, comprenant au moins un seul polymorphisme de nucléotide (SNP) et/ou au moins un polymorphisme d' oligonucléotide (OP) essentiellement propre au niveau du phylum à l'espèce des nématodes Méloïdogynes dont le nématode Méloïdogyne est l'un des membres, cette analyse d'acide nucléique requérant un bioessai d'hybridation. Ledit groupe comprend: (a) les espèces M. chitwoodi, M. fallax </i
Bibliography:Application Number: WO2007NL50343