AUTOMATABLE ARTIFICIAL IMMUNE SYSTEM (AIS)

• Main Authors: FAHLENKAMP, HEATHER, NGUYEN, MICHAEL, N, SANCHEZ-SCHMITZ, GUZMAN, MOE, DAVID, WARREN, WILLIAM, L, PARKHILL, ROBERT, HIGBEE, RUSSELL, KACHURIN, ANATOLY, M, DRAKE, DONALD, III
• Format: Patent
• Language: English; French
• Published: 22.11.2007
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. In particular the claimed artificial immune system comprises a vaccination site, comprising a first matrix and a plurality of cells attached to said matrix, wherein a plurality of said vaccination sites are organized in a multi-well format; and a three-dimensional artificial lymphoid tissue, comprising a second matrix and a plurality of lymphocytes and leukocytes, wherein a plurality of said three-dimensional artificial lymphoid tissues are organized in a second multi-well format. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system. Cette invention concerne des procédés permettant de construire un système immunitaire artificiel intégré comprenant des constructions tissulaires et cellulaires in vitro appropriées ou leurs équivalents pour imiter des tissus normaux qui interagissent avec les vaccins chez des mammifères. Le système immunitaire artificiel décrit dans cette invention peut être utilisé pour tester l'efficacité de vaccins potentiels in vitro, il permet d'accélérer la mise au point du vaccin et de tester les interactions des médicaments et des produits chimiques avec le système immunitaire.