DNA POLYMERASE BLENDS AND MUTANT DNA POLYMERASES

• Main Authors: MANDELMAN, DAVID, LEE, JUN, E, POTTER, ROBERT, JASON, ZHENG, WEIDONG
• Format: Patent
• Language: English; French
• Published: 04.01.2007
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; VINEGAR; WINE

A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5'-exonuclease activity and one of which has 5'-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis. Composition d'ADN polymérase thermostable comprenant au moins deux ADN polymérases, dont l'une est sensiblement réduite en termes d'activité exonucléase 5' et dont l'autre possède une activité exonucléase 5'. Cette polymérase peut être utilisée dans des méthodes telles que, mais sans s'y limiter, la synthèse d'acide nucléique, le séquençage de l'ADN, l'amplification d'acide nucléique et la synthèse de l'ADNc.