METHODS OF ACTIVATION DURING NUCLEAR TRANSFER

• Main Authors: GAVIN, WILLIAM, G, JELLERETTE, TERU
• Format: Patent
• Language: English; French
• Published: 26.07.2007
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

It has been found that caprine sperm factor, alone or in combination with adenophostin A, produces the Ca2+ oscillations characteristic of egg activation seen with fertilization. The introduction of caprine sperm factor and adenophostin A to eggs to produce reconstructed embryos has been optimized. On a trouvé que le facteur de sperme caprin, seul ou en combinaison avec l'adénophostine A, produisait les caractéristiques d'oscillations de Ca2+ de l'activation de l'oeuf envisagée avec la fertilisation. L'introduction du facteur de sperme caprin et d'adénophostine A dans des oeufs en vue de produire des embryons reconstruits a été optimisée.