PHOSPHOPROTEIN AFFINITY RESINS AND METHODS FOR MAKING AND USING THE SAME

• Main Authors: TCHAGA, GRIGORIY SIMEONOV, BHATIA, RAJINDER K
• Format: Patent
• Language: English; French
• Published: 20.04.2006
• Subjects: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; HUMAN NECESSITIES; HYGIENE; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; PEPTIDES; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SEPARATION; THEIR PREPARATION OR CHEMICAL WORKING-UP; THEIR RELEVANT APPARATUS; TRANSPORTING

Phosphorylated protein (i.e., phosphoprotein) affinity resins and methods for making and using the same are provided. The subject resins include a substrate bonded to aspartate-based tetradentate ligand/metal ion complexes, where the tetradentate ligand/metal ion complexes have high specificity for phosphorylated amino acids. The subject resins find use in a variety of different applications, including phosphoprotein enrichment applications. Also provided are kits and systems that include the subject resins. L'invention concerne des résines à affinité de protéines phosphorylées (par exemple, phosphoprotéines) et des procédés d'élaboration et d'utilisation correspondants. Les résines en question comprennent un substrat lié à des complexes ion métallique/ligand tétradentate à base d'aspartate, lesquels ont une forte spécificité pour les acides aminés phosphorylés. Les résines en question sont utilisées dans une série d'applications différentes, comme les applications d'enrichissement de phosphoprotéines. On décrit aussi des kits et des systèmes qui comportent les résines considérées.