RECOMBINANT CALF-CHYMOSIN AND A PROCESS FOR PRODUCING THE SAME

An isolated 1098 base-pair nucleic acid encoding a 366 amino acid calf chymosin is cloned in the E. coli expression vector PET21b, a host cell is transformed with the vector and cultured to produce chymosin, which is recovered in active form following renaturation and purification at low pH. Une chy...

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Main Authors GOPALA, KRISHNA, KARANAM, KAMALA, MYTHILI, PADALA, RAMANA, YAMALA, BOSU, REDDY, DONTHY, REDDY, VENKATA, MADHUSUDHAN, REDDY, MULE
Format Patent
LanguageEnglish
French
Published 09.04.2009
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMES
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:An isolated 1098 base-pair nucleic acid encoding a 366 amino acid calf chymosin is cloned in the E. coli expression vector PET21b, a host cell is transformed with the vector and cultured to produce chymosin, which is recovered in active form following renaturation and purification at low pH. Une chymosine de veau contenant 1098 p et codant pour 366 acides aminés a été isolée et clonée dans un vecteur PET 21b d'expression bactérienne de E.coli. On fait fermenter cette souche et la protéine est précipitée par adjonction d'alcali. Une forme active est extraite par renaturation et purifiée sous faible pH.
Bibliography:Application Number: WO2004IN00074