NUCLEIC ACID CONSTRUCT CONTAINING FULL-LENGTH GENOME OF HUMAN HEPATITIS C VIRUS, RECOMBINANT FULL-LENGTH VIRUS GENOME REPLICATIVE CELL HAVING THE NUCLEIC ACID CONSTRUCT TRANSFERRED THEREINTO AND METHOD OF CONSTRUCTING HEPATITIS C VIRAL PARTICLES

• Main Authors: WAKITA, TAKAJI, DATE, TOMOKO, MIYAMOTO, MICHIKO, KATO, TAKANOBU, TANABE, JUN-ICHI, SONE, SABURO
• Format: Patent
• Language: English; French; Japanese
• Published: 01.09.2005
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

It is intended to provide a method of efficiently replicating RNA containing the full-length genome sequence of HCV; a method of preparing viral particles of HCV containing full-length HCV replicon RNA or full-length HCV genome RNA by using a cell culture system; a method of producing hepatitis C viral particles which comprises culturing cells having replicon RNA comprising the full-length RNA sequence of hepatitis C virus of the genotype 2a and a base sequence containing at least one selection marker and/or at least one reporter gene and at least one IRES sequence or the full-length genome RNA of hepatitis C virus of the genotype 2a to thereby allowing the production of viral particles in the culture medium; a hepatitis C vaccine; and an antibody against hepatitis C viral particles. L'intention est de fournir un procédé de réplication efficace de l'ARN contenant la séquence du génome complet du VHC ; un procédé de préparation de particules virales de VHC contenant l'ARN réplicon du VHC complet ou l'ARN du génome complet du VHC en utilisant un système de culture de cellules ; un procédé de production de particules virales d'hépatite C qui comprend de mettre en culture des cellules ayant l'ARN réplicon comprenant la séquence complète de l'ARN du virus de l'hépatite C du génotype 2a et une séquence de base contenant au moins un marqueur de sélection et/ou au moins un gène reporteur et au moins une séquence IRES ou l'ARN du génome complet du virus de l'hépatite C du génotype 2a pour permettre de cette manière la production de particules virales dans le milieu de culture ; vaccin contre l'hépatite C ; et anticorps contre les particules virales d'hépatite C.