NOVEL GLYCOSYLTRANSFERASE, NUCLEIC ACID ENCODING THE SAME AND METHOD OF DETECTING CANCER TISSUE USING THE NCULEIC ACID

• Main Authors: NARIMATSU, HISASHI, TOGAYACHI, AKIRA, NISHIHARA, SHOKO, HIRUMA, TORU
• Format: Patent
• Language: English; French; Japanese
• Published: 13.05.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

It is intended to provide a novel glycosyltransferase, a nucleic acid encoding the same and a novel method of examining canceration of a tissue. The novel glycosyltransferase is 1,3-N-acetyl-D-glucosamine glycosyltransferase characterized by containing a polypeptide which has an amino acid sequence consisting of the 56- to 402-amino acids in SEQ ID NO:2 or an amino acid sequence derived from the above-described amino acid sequence by substitution, deletion or insertion of one or several amino acids and having an activity of transferring an N-acetyl-D-glucosamine group from an N-acetyl-D-glycosamine donor to an N-acetyl-D-glucosamine recipient. L'invention concerne une glycosyltransférase, un acide nucléique codant celle-ci et un procédé d'examen de la cancérisation d'un tissu. La glycosyltransférase est une 1,3-N-acétyl-D-glucosamine glycosyltransférase caractérisée en ce qu'elle renferme un polypeptide possédant une séquence d'acides aminés comprenant entre 56 et 402 acides aminés dans la SEQ ID NO : 2 ou une séquence d'acides aminés dérivée de la séquence d'acides aminés décrite ci-dessus par substitution, effacement ou introduction d'un ou de plusieurs acides aminés et possédant une activité permettant de transférer un groupe N-acétyl-D-glucosamine d'un donneur de N-acétyl-D-glycosamine à un récepteur de N-acétyl-D-glucosamine.