NOVEL CELLULASE TOLERANT TO SURFACTANTS

• Main Authors: YANAI, KOJI, OKAKURA, KAORU
• Format: Patent
• Language: English; French; Japanese
• Published: 13.05.2004
• Edition: 7
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; APPARATUS THEREFOR; BEER; BIOCHEMISTRY; BLEACHING LEATHER OR FURS; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DETERGENT COMPOSITIONS; DETERGENTS; DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; ENZYMOLOGY; FATTY ACIDS THEREFROM; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; IMPREGNATING OR COATING OF PAPER; LAUNDERING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PAPER NOT OTHERWISE PROVIDED FOR; PAPER-MAKING; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PRODUCTION OF CELLULOSE; PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; PULP COMPOSITIONS; RECOVERY OF GLYCEROL; REGENERATION OF PULPING LIQUORS; RESIN SOAPS; SOAP OR SOAP-MAKING; SPIRITS; TEXTILES; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; TREATMENT OF TEXTILES OR THE LIKE; USE OF SINGLE SUBSTANCES AS DETERGENTS; VINEGAR; WINE

A novel cellulase having an amino acid sequence derived from the amino acid sequence of cellulase NCE5 by substitution of the amino acid residues at the 162- and/or 166-positions by amino acids different from those of cellulase NCE5; a polynucleotide encoding this novel cellulase; an expression vector containing the same; host cells transformed by the expression vector; and a cellulase preparation and a detergent composition containing the above cellulase. This cellulase is tolerant to surfactants and sustains a high activity even under alkaline conditions. La présente invention se rapporte à: une nouvelle cellulase comprenant une séquence d'acides aminés dérivée de la séquence d'acides aminés de la cellulase NCE5, présentant une substitution de résidus d'acides aminés aux positions 162 et/ou 166 par des acides aminés différents de ceux de la cellulase NCE5; à un polynucléotide codant cette nouvelle cellulase; à un vecteur d'expression contenant cette dernière; à des cellules hôtes transformées par le vecteur d'expression précité; et à une préparation de cellulase et à une composition détergente contenant la cellulase de l'invention. La cellulase de l'invention est tolérante aux tensio-actifs et conserve une activité élevée même dans des conditions alcalines.