METHODS OF USE FOR THERMOSTABLE RNA LIGASES

• Main Authors: KRISTJANSSON, JAKOB, SKIRNISDOTTIR, SIGURLAUG, FRIDJONSSON, OLAFUR, H, BLONDAL, THORARINN, AEVARSSON, ARNTHOR, HREGGVIDSSON, GUDMUNDUR, OLI, HJORLEIFSDOTTIR, SIGRIDUR
• Format: Patent
• Language: English; French
• Published: 22.04.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The activity and functional properties of novel thermostable RNA ligases isolated from thermophilic bacteriophages are described. Also described are methods of using these thermostable RNA ligases for various applications including nucleotide labeling, oligonucleotide synthesis, gene synthesis, gene amplification and amplification of mRNA and synthesis of cDNA. La présente invention concerne l'activité et les propriétés fonctionnelles de nouvelles ligases d'ARN thermostables isolées à partir de bactériophages thermophiles. L'invention concerne également des procédés permettant d'utiliser ces ligases d'ARN thermostables pour diverses applications, parmi lesquelles, le marquage nucléotidique, la synthèse oligonucléotidique, la synthèse génique, l'amplification génique et l'amplification de l'ARNm et la synthèse de ADNc.