HIGH THROUGHPUT GENETIC SCREENING OF LIPID AND CHOLESTEROL PROCESSING USING FLUORESCENT COMPOUNDS

• Main Authors: PACK, MICHAEL, HALPERN, MARNIE, FARBER, STEVEN
• Format: Patent
• Language: English; French
• Published: 18.04.2002
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention utilizes fluorescent lipids, particularly quenched phospholipid or cholesterol analogues, to facilitate screening for phenotypes representing perturbations of lipid processing; screening for genetic mutations that lead to disorders of phospholipid and/or cholesterol metabolism; and screening of compounds designed to treat disorders of phospholipid and/or cholesterol metabolism. La présente invention concerne un procédé utilisant des lipides fluorescents, en particulier des analogues du cholestérol ou des phospholipides trempés, pour faciliter le criblage de phénotypes nuisant au traitement des lipides; le criblage des mutations génétiques entraînant des troubles du métabolisme phospholipidique et/ou du métabolisme du cholestérol; et le criblage de composés permettant de traiter les troubles du métabolisme phospholipidique et/ou du métabolisme du cholestérol.