NOVEL CLOCK GENE PROMOTER

• Main Authors: HASHIMOTO, SEIICHI, CHEN, WENBIN, TAKASUGI, TOMOHIRO
• Format: Patent
• Language: English; French; Japanese
• Published: 17.10.2002
• Edition: 7
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TESTING; TRAPPING; VINEGAR; WINE

A period 2 gene promoter; a construct containing this promoter and a reporter gene; cells containing this construct; a transgenic animal having the above construct integrated thereinto; and a method of screening a substance controlling the expression or the expression oscillation of a clock gene in vivo. In the above screening method, use is made of the above cells, a suprachiasmatic nucleus section or a peripheral tissue of the above transgenic animal or the transgenic animal per se. L'invention porte sur un promoteur du gène 2 de l'horloge interne ; un gène hybride contenant ce promoteur et un gène rapporteur ; des cellules contenant ce gène hybride ; un animal transgénique contenant le gène hybride susmentionné ; et sur un procédé de criblage d'une substance qui contrôle l'expression ou l'oscillation de l'expression d'un gène de l'horloge interne in vivo. Dans le procédé de criblage susmentionné, des cellules sont utilisées, ainsi qu'une section de noyau suprachiasmatique ou un tissu périphérique dudit animal transgénique ou l'animal transgénique en tant que tel.