SUSTAINED TOTIPOTENT REGENERABLE TISSUE CULTURE OF ARUNDO DONAX (GIANT REED) AND TOTIPOTENT TISSUE AND PLANTS PRODUCED THEREFROM

• Main Authors: MARTON, LASZLO, CZAKO, MIHALY
• Format: Patent
• Language: English; French
• Published: 15.08.2002
• Edition: 7
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATERTREATMENT OR WASTE MANAGEMENT; COMPOSITIONS THEREOF; CULTURE MEDIA; DISPOSAL OF SOLID WASTE; ENZYMOLOGY; FISHING; FORESTRY; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PERFORMING OPERATIONS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECLAMATION OF CONTAMINATED SOIL; RECLAMATION OF CONTAMINED SOIL SOIL; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; TRANSPORTING; TRAPPING; TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE; VINEGAR; WINE

A method is provided for generating sustained totipotent tissue cultures of giant reed (Arundo donax L.), and for micropropagating giant reed in vitro, wherein immature inflorescence are cultivated to produce totipotent tissue which is suitable for sustained maintenance and propagation. Greening of the tissue can be induced under light and the multishoot culture can multiply by microtillering. Foreign genes can be introduced into the tissue if desired, and the transgenic plants can be used in phytoremediation technologies in the field and in phytoreactors independently of seasons. L'invention concerne un procédé destiné à générer des cultures continues de tissu totipotent de canne de Provence (Arundo donax L.), et à assurer la micropropagation in vitro de la canne de Provence. Dans ce procédé, l'inflorescence immature est cultivée de manière à produire un tissu totipotent convenant au maintien durable et à la propagation continue. Le verdissage du tissu peut être induit par la lumière et la culture multi-pousses obtenue par microtallage. Si nécessaire, on peut introduire des gènes étrangers dans le tissu, et les plantes transgéniques peuvent être utilisées, indépendamment de la saison, dans des technologies de phytorémédiation en plein champ et dans de phytoréacteurs.