LAMINARIBIOSE PHOSPHORYLASE, PROCESS FOR PRODUCING THE SAME AND PROCESS FOR PRODUCING LAMINARIOLIGOSACCHARIDE BY USING THE ENZYME

• Main Authors: YAMADA, KOUHEI, OKAMOTO, KATSUYUKI, MIYOSHI, SHINSUKE
• Format: Patent
• Language: English; French; Japanese
• Published: 25.10.2001
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A laminarioligosaccharide is obtained by reacting thermotolerant laminaribiose phosphorylase obtained by culturing a microorganism belonging to the genus Bacillus with alpha -glucose-1-phosphate and glucose. A laminarioligosaccharide can be obtained too by adding starch and glucan phosphorylase as a substitute for alpha -glucose-1-phosphate. Thus, an inexpensive and safe process for producing a laminarioligosaccharide is provided. L'invention concerne un laminarioligosaccharide obtenu par réaction d'un phosphorylase de laminaribiose thermotolérant obtenu en cultivant un micro-organisme appartenant à l'espèce Bacillus avec alpha -glucose-1-phosphate et glucose. Un laminarioligosaccharide peut également être obtenu en ajoutant du phosphorylase d'amidon et de glycane en tant que substitut pour alpha -glucose-1-phosphate. Il en résulte un procédé sûr et peu coûteux de production d'un laminarioligosaccharide.