A GENERAL METHOD FOR OPTIMIZING THE EXPRESSION OF HETEROLOGOUS PROTEINS

• Main Author: BALINT, ROBERT, F
• Format: Patent
• Language: English; French
• Published: 26.04.2001
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Methods are disclosed whereby variants of proteins which do not confer selectable phenotypes can nevertheless be selected for stable expression in heterologous hosts. Related methods are disclosed whereby cDNA expression libraries can be enriched for stable expression of autonomously folding domains...