COMPOSITIONS FOR DNA AMPLIFICATION, SYNTHESIS, AND MUTAGENESIS

• Main Authors: MUHICH, MICHAEL, L, HOGREFE, HOLLY, HURLBUT, BORNS, MICHAEL, C
• Format: Patent
• Language: English; French
• Published: 12.04.2001
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

This invention provides compositions comprising a thermostable non-proofreading DNA polymerase, a thermostable proofreading DNA polymerase, and a factor that substantially inhibits the incorporation of undesired nucleotides or analogs thereof into a DNA polymer. The compositions may further comprise a buffer that enhances a polymerization reaction involving DNA polymerases. The invention also provides various methods of amplifying, synthesizing, or mutagenizing nucleic acids of interest using these novel compositions. Kits that comprise the compositions are also provided for amplifying, synthesizing, and mutagenizing nucleic acids. La présente invention concerne des compositions comprenant une polymérase d'ADN thermostable non correctrice, une polymérase d'ADN thermostable correctrice, et un facteur qui inhibe essentiellement l'incorporation de nucléotides indésirables ou analogues dans un polymère d'ADN. Les compositions peuvent également comprendre un tampon qui améliore la réaction de polymérisation faisant intervenir des polymérases d'ADN. Cette invention concerne également différents procédés permettant l'amplification, la synthèse et la mutagenèse d'acides nucléiques d'intérêt grâce aux compositions de l'invention. Cette invention concerne également des ensembles comprenant les compositions, servant à l'amplification, à la synthèse et à la mutagenèse d'acides nucléiques.