CHEMILUMINESCENT SUBSTRATES OF HYDROLYTIC ENZYMES SUCH AS PHOSPHATASES

• Main Authors: JIANG, QINGPING, SHARPE, DAVID, J, NATRAJAN, ANAND, LAW, SAY-JONG, WONG, WEN-JEE
• Format: Patent
• Language: English; French
• Published: 08.02.2001
• Edition: 7
• Subjects: ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM; ADHESIVES; APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; DYES; ENZYMOLOGY; HETEROCYCLIC COMPOUNDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; MUTATION OR GENETIC ENGINEERING; NATURAL RESINS; ORGANIC CHEMISTRY; PAINTS; PHYSICS; POLISHES; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

Chemiluminescent substrates of hydrolytic enzymes are disclosed having general Formula Lumi-M-P, where Lumi is a chemiluminescent moiety capable of producing light (a) by itself, (b) with MP attached and (c) with M attached, wherein the different properties of Lumi-M-P and Lumi-M allow them to be distinguished. Lumi includes, but is not limited to, acridinium compounds (e.g. acridinium esters, carboxyamides, thioesters and oxime esters), reduced forms thereof (e.g. acridans), and spiroacridan compounds. M is selected from oxygen, nitrogen and sulfur. P is a group that can be readily removed by hydrolytic enzymes to give Lumi-M and P. The hydrolytic enzyme can be phosphatase, glycosidase, peptidase, protease, esterase, sulfatase and guanidinobenzoatase. L'invention concerne des substrats chimiluminescents d'enzymes hydrolytiques représentés par la formule générale Lumi-M-P, dans laquelle Lumi est un fragment chimiluminescent capable de produire de la lumière (a) par lui-même, (b) avec MP et (c) avec M. Les différentes propriétés de Lumi-M-P et de Lumi-M permettent de les distinguer. Lumi comprend, entre autres, des composés d'acridinium chimiluminescents (par exemple des esters, des carboxyamides, des thioesters et des esters d'oxime d'acridinium), des formes réduites de ceux-ci (par exemple les acridanes) et des composés de spiroacridane. M est sélectionné parmi l'oxygène, le nitrogène et le soufre. P est un groupe pouvant être facilement retiré par des enzymes hydrolytiques pour obtenir Lumi-M et P. L'enzyme hydrolytique peut être la phosphatase, la glycosidase, la peptidase, la protéase, l'estérase, la sulfatase et la guanidinobenzoatase.