SUBTILISIN PROTEASE VARIANTS HAVING AMINO ACID DELETIONS AND SUBSTITUTIONS IN DEFINED EPITOPE REGIONS

• Main Authors: SIKORSKI, ELIZABETH, ELLEN, RUBINGH, DONN, NELTON
• Format: Patent
• Language: English; French
• Published: 07.03.2002
• Edition: 7
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; BEER; BIOCHEMISTRY; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DETERGENT COMPOSITIONS; DETERGENTS; ENZYMOLOGY; FATTY ACIDS THEREFROM; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECOVERY OF GLYCEROL; RESIN SOAPS; SOAP OR SOAP-MAKING; SPIRITS; USE OF SINGLE SUBSTANCES AS DETERGENTS; VINEGAR; WINE

The present invention relates to variants of subtilisin-like proteases having decreased immunogenicity relative to their corresponding wild-type proteases. The present invention further relates to such variants additionally having one or more amino acid substitutions in one or more epitope regions or additionally having one or more stabilizing substitutions. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants. L'invention concerne des variants de protéases subtilisinoïde, présentant une immunogénécité amoindrie par rapport à leurs protéases correspondantes de type sauvage. L'invention concerne également ces variants qui possèdent en plus, une ou plusieurs substitutions d'acide aminés, dans une ou plusieurs régions de sites antigéniques ou qui possèdent en plus, une ou plusieurs substitutions de stabilisation. L'invention concerne aussi les gènes mutants codant ces variants ainsi que des compositions de nettoyage et de soin personnel qui contiennent ces variants.