Method for dry packing chromatography columns

The invention provides a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a...

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Bibliographic Details
Main Authors Shanagar, Jamil, Widegren, Helena, Stridsberg-Friden, Kajsa, Lundgren, Bjorn, Hallgren, Elisabeth, Lind, Ola, Gebauer, Klaus, Lemmens, Raf
Format Patent
LanguageEnglish
Published 01.05.2018
Subjects
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
SEPARATION
TESTING
THEIR RELEVANT APPARATUS
TRANSPORTING
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Summary:The invention provides a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column.
Bibliography:Application Number: US201013518029