Automated immunoanalyzer system for performing diagnostic assays for allergies and autoimmune diseases

• Main Authors: Bao-Guey Chan Jennifer, Schell Rachel Sarah, Rieger Dennis Edwin, Sargeant Bruce Alan, Luo Yi, Knox Travis, Hall Eric Darnell, Canfield Douglas John, Morton John Lewis, Taylor Teri, Weston James, Moskalev Anatoly, Reid Taylor Addison, Hwang Tae Ho, Reliford Morkoah Blay, Trondle Linda Marie, Stack Marinela Gombosev, Diamond Ronald Norman, Gann Steve Michael, Sinson Edsel Lawrence Noche, Hung Victoria, Raghavan Nanditha, McMenamy Evan Phillip, Van Cleve Mark David, Ortega Stephanie TuVi, Wilson Kylie, Chua Vanessa Camille, Taine Elaine Grace, Vande Wetering Scott William, Forshager Michelle Fredrika, Cuaresma Jacalne Fran Zylo
• Format: Patent
• Language: English
• Published: 23.05.2017
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

A quantitative method for performing an automated diagnostic assay, comprising: incubating a capture reagent with a streptavidin-coated medium to form a solid phase complex; washing the solid phase complex to remove excess capture reagent; incubating the solid phase complex with a serum sample to form an immune complex; washing the immune complex to remove any unbound sample; incubating the immune complex with a conjugate to create an immune-conjugate complex; washing the immune-conjugate complex to remove any unbound conjugate; introducing a substrate capable of generating a quantifiable response; and calibrating the response generated from introducing the substrate.