Method for producing horseradish peroxidase recombinant protein using filamentous fungus

• Main Authors: Okakura Kaoru, Inoue Atsushi, Nagasato Toshiaki, Yanai Koji, Murashima Koichiro, Nakane Akitaka, Yokoyama Fumikazu
• Format: Patent
• Language: English
• Published: 10.01.2017
• Subjects: BEER; BIOCHEMISTRY; BLEACHING LEATHER OR FURS; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; ENZYMES; ENZYMOLOGY; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; LAUNDERING; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; TEXTILES; TREATMENT OF TEXTILES OR THE LIKE; VINEGAR; WINE

A modified polynucleotide has a different base sequence in at least one codon from a wild-type base sequence encoding a horseradish peroxidase polypeptide. The usage frequency of the modified codon of the polynucleotide corresponds to the codon usage frequencies of three filamentous fungal species in Humicola, Aspergillus, and Trichoderma. The polynucleotide is capable of expressing the polypeptide to be encoded in a filamentous fungus.