Pectin lyase, pectin lyase polynucleotide, enzyme preparation, and method for producing single cells of plant tissue

• Main Authors: UENOYAMA MITSUHIRO, KOBAYASHI ERINA, NAKANO YOSHIHISA
• Format: Patent
• Language: English
• Published: 08.04.2014
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; TRAPPING; VINEGAR; WINE

To provide a pectin lyase and a gene for a pectin lyase which can be used in an enzyme preparation for processing plant tissue, and which possesses sufficient maceration activity even under highly acidic conditions. Isolation and purification of a pectin lyase and a gene for a pectin lyase having a molecular weight of 39,500 produced by an Aspergillus filamentous fungus, and possessing a maceration activity and a pectin lyase activity. The pectin lyase has a specified amino acid sequence, an optimal pH of 3.5 for maceration activity, an optimal pH of 4.5 for pectin lyase activity, and deactivates with boiling treatment for 15 minutes at 121° C. Sterilizing heat treatment are unnecessary, because the present invention possesses sufficient maceration activity at pH 3.0-4.0, and the resulting pectin lysate possesses a bactericidal activity and a potent antibacterial activity. It is possible to inexpensively mass produce food products which retain water-soluble components within the cell, because physical treatment such as stirring or beating is also unnecessary.