Methods for purifying nucleic acids
Methods are provided for producing highly purified compositions of nucleic acids by using a highly streamlined and readily automated process. The methods use static mixers for lysing cells and precipitating debris, following by centrifugation and ion exchange chromatography. The process may include...
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Main Authors | , , |
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Format | Patent |
Language | English |
Published |
25.03.2014
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Subjects | |
Online Access | Get full text |
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Summary: | Methods are provided for producing highly purified compositions of nucleic acids by using a highly streamlined and readily automated process. The methods use static mixers for lysing cells and precipitating debris, following by centrifugation and ion exchange chromatography. The process may include a purification step using tangential flow ultrafiltration. A scaleable process for producing pharmaceutical grade plasmid DNA, useful for gene therapy, is provided. |
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Bibliography: | Application Number: US20100796961 |