Methods of interferon- purification and recovery

Improved methods for purification and recovery of interferon-beta (IFN- ) and compositions comprising substantially monomeric IFN- are provided. In one purification method, substantially purified IFN- or variant thereof is precipitated and then dissolved in a guanidine hydrochloride (HCl) solution....

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Bibliographic Details
Main Authors ESIKOVA IRINA, FORDHAM DENNIS, SHIRLEY BRET A, BABUKA SUSAN, WOLFE SIDNEY N
Format Patent
LanguageEnglish
Published 05.03.2013
Subjects
CHEMISTRY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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Summary:Improved methods for purification and recovery of interferon-beta (IFN- ) and compositions comprising substantially monomeric IFN- are provided. In one purification method, substantially purified IFN- or variant thereof is precipitated and then dissolved in a guanidine hydrochloride (HCl) solution. Renaturation of the protein occurs by dilution with a suitable buffer. A similar purification method absent the precipitation step is also provided. Following renaturation of the IFN- , residual guanidine HCl is removed by diafiltration or dialysis with a pharmaceutically acceptable buffer to prepare pharmaceutical compositions comprising substantially monomeric IFN- .
Bibliography:Application Number: US20080150530