Method of producing an immunogenic composition
The present invention relates to a method for purifying bacterial cytolysins such as pneumococcal pneumolysin. A single chromatography step produces excellent purification of the cytolysin by binding soluble aggregated cytolysin to a hydrophobic interaction chromatography material in the presence of...
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Main Authors | , , , |
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Format | Patent |
Language | English |
Published |
13.11.2012
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Subjects | |
Online Access | Get full text |
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Summary: | The present invention relates to a method for purifying bacterial cytolysins such as pneumococcal pneumolysin. A single chromatography step produces excellent purification of the cytolysin by binding soluble aggregated cytolysin to a hydrophobic interaction chromatography material in the presence of detergent and high salt. |
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Bibliography: | Application Number: US201113222066 |