Method for identifying gene function

Methods are provided in which a promoter hairpin construct is introduced into a cell to suppress expression of a gene of interest; a change in phenotype of the cell, or of an organism regenerated from the cell, is detected; and the change in phenotype identifies the function of a product of the gene...

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Bibliographic Details
Main Authors CIGAN ANDREW MARK, UNGER ERICA
Format Patent
LanguageEnglish
Published 23.10.2012
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DERIVATIVES THEREOF
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
SUGARS
TRAPPING
VINEGAR
WINE
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Summary:Methods are provided in which a promoter hairpin construct is introduced into a cell to suppress expression of a gene of interest; a change in phenotype of the cell, or of an organism regenerated from the cell, is detected; and the change in phenotype identifies the function of a product of the gene of interest. Also provided are methods for restoring the original phenotype.
Bibliography:Application Number: US20080061834