Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher eucaryotic cells using electrical current

• Main Authors: LORBACH ELKE, WEIGEL MEIKE, MUELLER-HARTMANN HERBERT, WESSENDORF HEIKE, THIEL CORINNA, RIEMEN GUDULA, SIEBENKOTTEN GREGOR, CHRISTINE RAINER, ALTROGGE LUDGER, HELFRICH JULIANA, ROTHMANN-COSIC KIRSTEN
• Format: Patent
• Language: English
• Published: 08.05.2012
• Subjects: APPARATUS FOR CONVERSION BETWEEN AC AND AC, BETWEEN AC AND DC,OR BETWEEN DC AND DC, AND FOR USE WITH MAINS OR SIMILAR POWERSUPPLY SYSTEMS; APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BASIC ELECTRIC ELEMENTS; BASIC ELECTRONIC CIRCUITRY; BEER; BIOCHEMISTRY; BLASTING; CHEMISTRY; COMPOSITIONS THEREOF; CONTROL OR REGULATION THEREOF; CONVERSION OF DC OR AC INPUT POWER INTO SURGE OUTPUTPOWER; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER; CORONA DEVICES; CULTURE MEDIA; ELECTRICITY; ELECTROTHERAPY; ENZYMOLOGY; GENERATING IONS TO BE INTRODUCED INTO NON-ENCLOSED GASES; GENERATION; HEATING; HUMAN NECESSITIES; HYGIENE; LIGHTING; MAGNETOTHERAPY; MECHANICAL ENGINEERING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; OVERVOLTAGE ARRESTERS USING SPARK GAPS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; PULSE TECHNIQUE; RADIATION THERAPY; SPARK GAPS; SPARKING PLUGS; SPIRITS; ULTRASOUND THERAPY; VINEGAR; WEAPONS; WEAPONS FOR PROJECTING MISSILES WITHOUT USE OF EXPLOSIVE ORCOMBUSTIBLE PROPELLANT CHARGE; WEAPONS NOT OTHERWISE PROVIDED FOR; WINE

The invention relates to a novel circuit arrangement for electrotransfection or electrofusion, which enables the transportation of DNA and/or other biologically active molecules to the nucleus of higher eukaryotic cells or the fusion of cells, independent of cell division and with reduced cell mortality.