Sugar-immobilized metal nanoparticle, method for measuring sugar-protein interaction using the same and method for recovering protein from sugar-protein interactant

• Main Authors: KISHIMOTO YUKO, NAKAGAWA HIROMI, NISHIMURA TOMOAKI, SUDA YASUO
• Format: Patent
• Language: English
• Published: 29.11.2011
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; NANOTECHNOLOGY; ORGANIC CHEMISTRY; PEPTIDES; PERFORMING OPERATIONS; PEST REPELLANTS OR ATTRACTANTS; PHYSICS; PLANT GROWTH REGULATORS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; SACCHARIDES, OTHER THAN SUCROSE, OBTAINED FROM NATURAL SOURCESOR BY HYDROLYSIS OF NATURALLY OCCURRING DI-, OLIGO- ORPOLYSACCHARIDES; SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; SUGAR INDUSTRY; TESTING; TRANSPORTING; TRAPPING

It is intended to provide a stable novel sugar-immobilized metal nanoparticle capable of easily immobilizing a sugar chain, a method for measuring sugar-protein interaction easily and at a low cost using the same without labeling, and a method for simply recovering a protein from a sugar-protein interactant. A maltose-immobilized gold nanoparticle was obtained by binding a ligand complex, in which maltose and a linker compound had been bound to each other, to a gold nanoparticle. By adding this maltose-immobilized gold nanoparticle to a dilution series of concanavalin A, a sugar-protein interactant of maltose and ConA was formed, and red-purple color derived from a colloidal solution of maltose-immobilized gold nanoparticle disappeared. That is, sugar-protein interaction could be confirmed by visual observation without labeling.