Modified beta-lactamase and method for its preparation
The invention relates to targeted post translational modification of metallo-beta-lactamase by truncation and insertion of a dipeptide at the amino terminal end to reduce amino terminal heterogeneity in a recombinant DNA production system. A protein K-T-E- BL is expressed, and modified by host prote...
Saved in:
Main Authors | , , |
---|---|
Format | Patent |
Language | English |
Published |
02.08.2011
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | The invention relates to targeted post translational modification of metallo-beta-lactamase by truncation and insertion of a dipeptide at the amino terminal end to reduce amino terminal heterogeneity in a recombinant DNA production system. A protein K-T-E- BL is expressed, and modified by host proteases to E- BL. Appropriate nucleotide molecules, vectors and hosts are also described. E- BL is useful in a pharmaceutical composition for treating antibiotic induced adverse effects in the intestine of patients treated with beta-lactam antibiotics. |
---|---|
AbstractList | The invention relates to targeted post translational modification of metallo-beta-lactamase by truncation and insertion of a dipeptide at the amino terminal end to reduce amino terminal heterogeneity in a recombinant DNA production system. A protein K-T-E- BL is expressed, and modified by host proteases to E- BL. Appropriate nucleotide molecules, vectors and hosts are also described. E- BL is useful in a pharmaceutical composition for treating antibiotic induced adverse effects in the intestine of patients treated with beta-lactam antibiotics. |
Author | KOSKI PERTTI KAEAERIAEINEN SUSANNA WICKSTRAND NINA |
Author_xml | – fullname: WICKSTRAND NINA – fullname: KOSKI PERTTI – fullname: KAEAERIAEINEN SUSANNA |
BookMark | eNrjYmDJy89L5WQw881PyUzLTE1RSEotSdTNSUwuScxNLE5VSMxLUchNLcnIT1FIyy9SyCwpVigoSi1ILEosyczP42FgTUvMKU7lhdLcDApuriHOHrqpBfnxqcUFicmpeakl8aHB5pYWloaWRk5GxkQoAQAzZy8M |
ContentType | Patent |
DBID | EVB |
DatabaseName | esp@cenet |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: EVB name: esp@cenet url: http://worldwide.espacenet.com/singleLineSearch?locale=en_EP sourceTypes: Open Access Repository |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Chemistry Sciences |
ExternalDocumentID | US7989192B2 |
GroupedDBID | EVB |
ID | FETCH-epo_espacenet_US7989192B23 |
IEDL.DBID | EVB |
IngestDate | Fri Jul 19 14:49:36 EDT 2024 |
IsOpenAccess | true |
IsPeerReviewed | false |
IsScholarly | false |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-epo_espacenet_US7989192B23 |
Notes | Application Number: US20070304874 |
OpenAccessLink | https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20110802&DB=EPODOC&CC=US&NR=7989192B2 |
ParticipantIDs | epo_espacenet_US7989192B2 |
PublicationCentury | 2000 |
PublicationDate | 20110802 |
PublicationDateYYYYMMDD | 2011-08-02 |
PublicationDate_xml | – month: 08 year: 2011 text: 20110802 day: 02 |
PublicationDecade | 2010 |
PublicationYear | 2011 |
RelatedCompanies | IPSAT THERAPIES OY |
RelatedCompanies_xml | – name: IPSAT THERAPIES OY |
Score | 2.8111193 |
Snippet | The invention relates to targeted post translational modification of metallo-beta-lactamase by truncation and insertion of a dipeptide at the amino terminal... |
SourceID | epo |
SourceType | Open Access Repository |
SubjectTerms | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE |
Title | Modified beta-lactamase and method for its preparation |
URI | https://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20110802&DB=EPODOC&locale=&CC=US&NR=7989192B2 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LS8NAEB5KFfWmVbG-2IPkFox55xCEvChC2mIb6a1kNxsIaBKaiH_f2bSpXvS27MIwuzAzHzM73wA8MGYxzc4VmZsOlXWemTKldo5AzjKoJSjVbdEoHE_NSaK_rIzVAIq-F6bjCf3qyBHRohjae9v56_oniRV0fyubR1rgVvUcLd1Ayvp0n2gdlQLPDeezYOZLvu8mC2n66lqO7SCY8dBbHwgULWj2wzdPNKXUvyNKdAqHcxRWtmcw4OUIjv1-8NoIjuJdvRuXO9NrzsGMq6zIETASiprJ7ylr0w8MQSQtM7KdA00QgJKibUi94VtK76q8ABKFS38iowLr_WXXyWKvqnYJw7Iq-RUQFV9SNTKN5wbTldSxFTN1coeJqlz6xIwxjP8Uc_3P2Q2c9ElSRb2FYbv55HcYZVt6373PN7Dfgh4 |
link.rule.ids | 230,309,786,891,25594,76906 |
linkProvider | European Patent Office |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LS8NAEB5KFetNq2J97kFyC8a2eR2CkKQhapMW20hvJbvZQECT0kT8-86mTfWit2UXhtmFmfmY2fkG4I4xnQ2MVJG5ZlJ5yBNNptRIEcjpKtUFpbohGoWDUPOj4fNCXbQga3phap7Qr5ocES2Kob1Xtb9e_SSx3PpvZXlPM9wqHr255UpJk-4TraOSa1uj6cSdOJLjWNFMCl8t3TRMBDM2eus9XZDzCuT0ZoumlNXviOIdwf4UheXVMbR43oWO0wxe68JBsK1343JreuUJaEGRZCkCRkJRM_k9ZlX8gSGIxHlCNnOgCQJQklUlWa35htK7yE-BeKO548uowHJ32WU026k6OIN2XuT8HEgfX7KvJgOeqmyoxKahaLGZmkxU5eIHpvag96eYi3_ObqHjz4PxcvwUvlzCYZMwVfpX0K7Wn_waI25Fb-q3-gbvLoUL |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Apatent&rft.title=Modified+beta-lactamase+and+method+for+its+preparation&rft.inventor=WICKSTRAND+NINA&rft.inventor=KOSKI+PERTTI&rft.inventor=KAEAERIAEINEN+SUSANNA&rft.date=2011-08-02&rft.externalDBID=B2&rft.externalDocID=US7989192B2 |