Modified beta-lactamase and method for its preparation
The invention relates to targeted post translational modification of metallo-beta-lactamase by truncation and insertion of a dipeptide at the amino terminal end to reduce amino terminal heterogeneity in a recombinant DNA production system. A protein K-T-E- BL is expressed, and modified by host prote...
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Main Authors | , , |
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Format | Patent |
Language | English |
Published |
02.08.2011
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Subjects | |
Online Access | Get full text |
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Summary: | The invention relates to targeted post translational modification of metallo-beta-lactamase by truncation and insertion of a dipeptide at the amino terminal end to reduce amino terminal heterogeneity in a recombinant DNA production system. A protein K-T-E- BL is expressed, and modified by host proteases to E- BL. Appropriate nucleotide molecules, vectors and hosts are also described. E- BL is useful in a pharmaceutical composition for treating antibiotic induced adverse effects in the intestine of patients treated with beta-lactam antibiotics. |
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Bibliography: | Application Number: US20070304874 |