Method for making beta-secretase co-crystals complexed with a ligand

The present application relates to methods for growing crystals of both the uncomplexed and complexed forms of beta-secretase (BACE) polypeptide. The present application also relates to crystalline forms of uncomplexed BACE and the three-dimensional structure of BACE, as determined from the crystals...

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Bibliographic Details
Main Authors WANG WENYAN, WILSON EILEEN, MALCOLM BRUCE A, STRICKLAND COREY O, BEYER BRIAN M
Format Patent
LanguageEnglish
Published 04.08.2009
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:The present application relates to methods for growing crystals of both the uncomplexed and complexed forms of beta-secretase (BACE) polypeptide. The present application also relates to crystalline forms of uncomplexed BACE and the three-dimensional structure of BACE, as determined from the crystals. In addition, the present application relates to the use of crystalline forms of BACE to identify ligands, preferably inhibitors (antagonists), which bind to, and preferably inhibit the enzymatic activity of, BACE. Furthermore, the present application relates to nucleic acid sequences encoding human BACE polypeptide, and methods for making BACE in greater quantity than prior methods, resulting in more effective crystallization.
Bibliography:Application Number: US20060634737