Genes encoding novel proteolytic enzymes

• Main Authors: WAGNER CHRISTIAN, MAIER DIETER, TAN PAMELA, KRUBASIK PHILIPP, GUSTEDT WILK VON, KIMPEL ERIK, EDENS LUPPO, KLUGBAUER SABINE, FRITZ ANDREAS, STOCK ALEXANDER, SPREAFICO FABIO, ALBERMANN KAJ, DIJK ALBERTUS VAN, FOLKERS ULRIKE, HOPPER SYLVIA, KEMMNER WOLFRAM, HEINRICH OLIVER, ALBANG RICHARD, STIEBLER JOSEPHINE
• Format: Patent
• Language: English
• Published: 29.01.2008
• Subjects: AGEING; BEER; BIOCHEMISTRY; CHEMISTRY; CLARIFICATION; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PASTEURISATION; PEPTIDES; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS; PHYSICS; PRESERVATION; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; PROTEIN COMPOSITIONS FOR FOODSTUFFS; PURIFICATION; SPIRITS; STERILISATION; SUGARS; TESTING; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE; WORKING-UP PROTEINS FOR FOODSTUFFS

The invention relates to newly identified gene sequences that encode novel proteases obtainable from Aspergillus niger. The invention features the full length gene sequence of the novel genes, their cDNA sequences as well as the full-length functional protein and fragments thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with DNA according to the invention and cells wherein a protease according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.