Method of depositing S-layer proteins on a carrier to immobilize functional molecules

• Main Authors: SLEYTR UWE B, SARA MARGIT, KAUTEK WOLFGANG
• Format: Patent
• Language: English
• Published: 28.08.2007
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS; TESTING; VINEGAR; WINE

On a carrier surface, a layer of S-layer proteins is produced as a carrier of functional molecules. A solution containing S-layer proteins in the form of monomers or oligomers is brought into contact with the carrier surface. Electrochemical conditions are produced in the solution such that the S-layer proteins (SU) have an electrical net charge by establishing an electrochemical potential difference between the solution and the carrier surface such that the S-layer proteins accumulate on the carrier surface. A two-dimensional crystalline structure is formed in the layer, and this can occur at a time separate from the deposition of the S-layer proteins, and under different electrochemical conditions.